Fluorescence Microscopy Method for Protein Counting

Stepwise Photobleaching for Accurate Analysis

In the realm of cellular biology, understanding the intricacies of protein dynamics is crucial. Researchers have devised a revolutionary fluorescence microscopy technique known as Protein-tag Degree of Labeling (ProDOL) for precisely counting protein molecules.

ProDOL: A Powerful Tool for Quantitative Protein Analysis

ProDOL employs stepwise photobleaching, a method that involves gradually bleaching fluorescently tagged proteins to measure their abundance. This approach offers an unparalleled dynamic range of over three orders of magnitude, enabling researchers to accurately determine the number of proteins in a given sample.

Unlocking Cellular Mechanisms

By precisely quantifying protein levels, ProDOL provides valuable insights into cellular functions. It allows scientists to explore protein expression dynamics in response to various stimuli, study protein degradation pathways, and uncover the molecular mechanisms underlying cellular processes.